Development of a reverse transcription loop-mediated isothermal amplification assay for detection of Porcine teschovirus
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چکیده
منابع مشابه
Reverse transcription loop-mediated isothermal amplification assay for rapid detection of Bovine Rotavirus
BACKGROUND Bovine rotavirus (BRV) infection is common in young calves. This viral infection causes acute diarrhea leading to death. Rapid identification of infected calves is essential to control BRV successfully. Therefore development of simple, highly specific, and sensitive detection method for BRV is needed. RESULTS A reverse transcription loop-mediated isothermal amplification (RT-LAMP) ...
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The fragment of the membrane protein M gene with high conservation and specificity of porcine reproductive and respiratory syndrome virus (PRRSV) was chosen to be the target region, according to which six special primers were designed successfully. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) was applied to detect the PRRSV by incubation at 65° for only 45 min with the...
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ژورنال
عنوان ژورنال: Journal of Veterinary Diagnostic Investigation
سال: 2011
ISSN: 1040-6387,1943-4936
DOI: 10.1177/1040638711403427